Plant Biotechnology and Tissue Culture
1. What is "totipotency"? The ability of a plant cell to perform all the activities of development like a zygote to develop into a complete plant is termed as totipotency. Haberlandt ,1902 first demonstrated totipotency by in vitro technique. 2. How do haploid plants produced? Haploid (n) plants are obtained by induction of embryogenesis during Anther and Pollen Culture. 3. How are haploid plants made Homozygous diploid ? 2/2020 BotH,TU The chromosome set of haploid pollen grains are first doubled by mutagenic chemicals like colchine (0.5% for 24-48 hr.). Then homokaryotic fusion resulted into production of homozygous diploid plants ( Vasil and Nitsch,1975). 4. What is Anther Culture?Describe the process with factors. Th...